wizard dna cleanup kit Search Results


t1030 kit  (New England Biolabs)
99
New England Biolabs t1030 kit
T1030 Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t1030 kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
t1030 kit - by Bioz Stars, 2026-03
99/100 stars
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dna cleanup micro kit  (Thermo Fisher)
95
Thermo Fisher dna cleanup micro kit
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Dna Cleanup Micro Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna cleanup micro kit/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
dna cleanup micro kit - by Bioz Stars, 2026-03
95/100 stars
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monarch spin pcr dna cleanup kit  (New England Biolabs)
99
New England Biolabs monarch spin pcr dna cleanup kit
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Monarch Spin Pcr Dna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monarch spin pcr dna cleanup kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
monarch spin pcr dna cleanup kit - by Bioz Stars, 2026-03
99/100 stars
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monarch pcr dna cleanup kit  (New England Biolabs)
99
New England Biolabs monarch pcr dna cleanup kit
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Monarch Pcr Dna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monarch pcr dna cleanup kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
monarch pcr dna cleanup kit - by Bioz Stars, 2026-03
99/100 stars
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monarch spin high capacity dna cleanup kit  (New England Biolabs)
93
New England Biolabs monarch spin high capacity dna cleanup kit
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Monarch Spin High Capacity Dna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monarch spin high capacity dna cleanup kit/product/New England Biolabs
Average 93 stars, based on 1 article reviews
monarch spin high capacity dna cleanup kit - by Bioz Stars, 2026-03
93/100 stars
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mo bio powerclean dna cleanup kit for sequencing  (Qiagen)
95
Qiagen mo bio powerclean dna cleanup kit for sequencing
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Mo Bio Powerclean Dna Cleanup Kit For Sequencing, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mo bio powerclean dna cleanup kit for sequencing/product/Qiagen
Average 95 stars, based on 1 article reviews
mo bio powerclean dna cleanup kit for sequencing - by Bioz Stars, 2026-03
95/100 stars
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wizard genomic dna purification kit  (Promega)
90
Promega wizard genomic dna purification kit
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizard genomic dna purification kit/product/Promega
Average 90 stars, based on 1 article reviews
wizard genomic dna purification kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
kits for dna purification  (Promega)
90
Promega kits for dna purification
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Kits For Dna Purification, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kits for dna purification/product/Promega
Average 90 stars, based on 1 article reviews
kits for dna purification - by Bioz Stars, 2026-03
90/100 stars
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wizard sv genomic dna purification system (promega, madison, wi, usa); cat. a1120  (Promega)
90
Promega wizard sv genomic dna purification system (promega, madison, wi, usa); cat. a1120
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Wizard Sv Genomic Dna Purification System (Promega, Madison, Wi, Usa); Cat. A1120, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizard sv genomic dna purification system (promega, madison, wi, usa); cat. a1120/product/Promega
Average 90 stars, based on 1 article reviews
wizard sv genomic dna purification system (promega, madison, wi, usa); cat. a1120 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
dna wizard kit  (Promega)
90
Promega dna wizard kit
( A ) Top: Domain architecture of E. coli <t>DNA</t> gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in <t>the</t> <t>purified</t> QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.
Dna Wizard Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna wizard kit/product/Promega
Average 90 stars, based on 1 article reviews
dna wizard kit - by Bioz Stars, 2026-03
90/100 stars
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( A ) Top: Domain architecture of E. coli DNA gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in the purified QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.

Journal: bioRxiv

Article Title: Pentapeptide repeat proteins QnrB1 and AlbG require ATP hydrolysis to rejuvenate poisoned gyrase complexes

doi: 10.1101/2020.09.24.310243

Figure Lengend Snippet: ( A ) Top: Domain architecture of E. coli DNA gyrase. Gyrase B is composed of ATPase, transducer (both purple) and TOPRIM (pink) domains. Indicated are molecular masses of different protein fragments (24 kDa, 43 kDa and 47 kDa) produced and used in this work. Gyrase A is composed of winged-helix (WHD), tower, coiled-coil (all three blue) and β – pinwheel (cyan) domains. Below : A scheme of DNA gyrase mechanism. ( 1 ) A double-stranded DNA (G-segment (red)) is captured by the DNA gate, the ATPase gate is open to allow enter of the T-segment (yellow); ( 2 ) ATP binding leads to dimerization of the ATPase domains and trapping of the T-segment in the upper cavity; ( 3 ) this is followed by ATP hydrolysis, G-segment cleavage, DNA-gate opening and T-segment translocation. ( B ) Cartoon and molecular surface (transparent) representations of two PRPs used in this study – QnrB1 PDB: 2XTW ( top ) and AlbG PDB: 2XT2 ( bottom ). Indicated are looped regions, previously found to be implicated in protective activity. ( C ). Plasmid supercoiling assay showing: ( left ) lane 1: relaxed pBR322, lane 2: supercoiling by 1 U of gyrase, lane 3: lack of detectable nuclease activity in the purified QnrB1 (50 μM QnrB1) and lane 4: partial inhibition of gyrase by 50 μM QnrB1; ( right ) lanes 6-13: gyrase inhibition by CFX (5 μM) and rescue by increasing concentrations of QnrB1 (0.04; 0.2; 1; 5; 10; 20; 25; 50 μM). Positions of negatively supercoiled and relaxed DNA are indicated by the graphics at the left (same notation used in other figures). ( D ) Plasmid supercoiling assay showing ( left ): lane 1: relaxed pBR322; lane 2: supercoiling by 1 U of gyrase; lane 3: lack of detectable nuclease activity in the purified AlbG (50 μM) and lane 4: lack of effect of 50 μM AlbG on gyrase supercoiling; ( right ) gyrase inhibition by ALB (3.3 μM) and partial rescue by increasing concentrations of AlbG (0.0016; 0.008; 0.04; 0.2; 1; 5; 10; 20; 25; 50 μM) ( E ). Left gel shows gyrase supercoiling inhibition by increasing concentrations of albicidin lanes 1-5 (0.016; 0.16; 1.6; 16; 160 [μM]). Right gel, effect of the addition of 5 μM QnrB1 to the same reactions (lanes 6 – 10) ( F ). Left , lanes 1-5: gyrase supercoiling inhibition by increasing concentrations of ciprofloxacin (0.016; 0.08; 0.4; 2; 10 μM); right , lanes 6 – 10: effect of adding 5 μM AlbG to the reactions.

Article Snippet: 147 bp pBR322 dsDNA fragment with known strong gyrase binding site ( ) was produced by PCR and purified with Thermo Scientific GeneJet Gel Extraction and DNA Cleanup Micro kit.

Techniques: Produced, Binding Assay, Translocation Assay, Activity Assay, Plasmid Preparation, Purification, Inhibition